1. Freeze tissue in liquid nitrogen after excision
2. Store at -80oC until needed
3. Add some liquid nitrogen to the tissue in a mortar
4. Powder frozen tissue with mortar and pestle
5. Add RIPA buffer to powdered tissue
6. Vortex 60 seconds
7. Put on ice for 45 minutes
8. Homogenize with a polytron (2 x 15 seconds)
9. Centrifuge at 14 000 x G for 10 minutes at 4oC
10. The samples are now ready to be used; or they can be stored at -80oC until needed