western blot protocols, troubleshooting, scientific resources and research atricles for the research community and science students


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Acrylamide Gel Preparation      
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 		   Resolving gel  
30% Bis/Acrylamide
1.5 M Tris-HCl pH8.8
10% SDS (pH 7.2)
nanopure H2O
10% APS
TEMED		 5 ml
5 ml
0.2 ml
9.7 ml
100 µl
10 µ		  


1. PubMed: comprehensive database - 15 million citations

2. NIH:the National Institutes of Health

3. UniProt: The world's most comprehensive catalogue of information on proteins

4. Western Blot Procedure Western blot protocol overview.

 

  
Stacking gel
30% Bis/Acrylamide
1 M Tris-HCl pH 6.8
10% SDS (pH 7.2)
nanopure H2O
10% APS
TEMED		 1.3 ml
1 ml
80 µl
5.5 ml
80 µl
8 µl
 
   
         
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