Table 1
Set up of the Taguchi optimisation experiment presented in Figure 2 with total number of spots detected in each gel.
Buffer*
 		Ampholytes(%)
 		CHAPS(%)
 		ASB14(%)
 		DTT(mM)
 		Spot number**
1
 		0.5
 		0.5
 		0.4
 		20
 		361
2
 		0.5
 		1.0
 		0.8
 		40
 		339
3
 		0.5
 		2.0
 		1.6
 		80
 		339
4
 		1.0
 		0.5
 		0.8
 		80
 		296
5
 		1.0
 		1.0
 		1.6
 		20
 		351
6
 		1.0
 		2.0
 		0.4
 		40
 		355
7
 		2.0
 		0.5
 		1.6
 		40
 		319
8
 		2.0
 		1.0
 		0.4
 		80
 		327
9
 		2.0
 		2.0
 		0.8
 		20
 		299
* – all buffers contained 7 M urea, 2 M thiourea, 10 mM Tris
** – number of spots detected, using Phoretix 2D Pro imaging software