| Rapid, stable suppresion of NDR by
retrovirus-delivered siRNA HeLa cells were infected with the viruses
illustrated in Fig. 1. Proteins were extracted from cells infected with
each virus and NDR levels were examined by western blot analysis. Arrow
indicates dramatic downregulation of NDR only in cells infected with MSCV/U6-NDR.
The upper band was detected by the anti-p75 antibody, which serves as an
internal loading and specificity control. Identical results were obtained
with cells examined 24-hours post infection or after several passages (data
not shown). |
