| FAK and paxillin immunoprecipitate with
β1 integrin following cell attachment to
type IV collagen. SCC25 cells were plated in semisolid media (susp), on
tissue culture plastic (pl), or on type IV collagen (col) for 2 hours. Some
cultures were preincubated with antibodies which block attachment by β1
or β4 integrin subunits. β1
integrin was immunoprecipitated (IP β1)
from cell lysates and western blotted using the antibodies indicated at
left. These experiments were performed three times with similar results.
Representative blots are shown. (B) Densitometric quantitation of blots
in Fig. 1A. |
