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Coomassie Blue Staining      
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Coomassie Brilliant Blue R250, binds nonspecifically to virtually all proteins. Coomassie Blue staining is less sensitive than silver staining, but effective nonetheless - in addition to being easy to perform. After staining the PVDF membrane, wash the membrane in destaining solution to remove excess dye.

  

1. PubMed: comprehensive database - 15 million citations

2. NIH:the National Institutes of Health

3. UniProt: The world's most comprehensive catalogue of information on proteins

4. Western Blot Procedure Western blot protocol overview.

 

 Coomassie Brilliant Blue Stain:  
Coomassie Brilliant Blue R250
 0.25 g
MetOH:H2O (1:1 v/v)
 90 ml
  Glacial acetic acid 10 ml
Procedure:
a. Mix the protein stain components listed above; filter the solution using a Whatman No. 1 filter.

b. Soak the gel in the dye solution for 30 min.

c. Excess Coomassie stain will be washed out during destaining (30 min).

d. The destaining solution is prepared as above - without the Coomassie R250 dye powder.
       
           
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